期刊
INTERNATIONAL JOURNAL OF FOOD MICROBIOLOGY
卷 133, 期 3, 页码 225-233出版社
ELSEVIER
DOI: 10.1016/j.ijfoodmicro.2009.05.005
关键词
Bacteriocin; Listeria monocytogenes; Pediococci; Animal model
资金
- Natural Sciences and Engineering Research Council of Canada (NSERC)
- Swiss National Foundation [3100A0-102256]
- Fonds Quebecoise de la Recherche sur la Nature et les Technologies (FQRNT)
The anti-listerial effect of pediocin PA-1 and its producing strain, Pediococcus acidilactici UL5, was investigated in vivo using an ICR mouse model. The effect of intra-gastric administration of a single dose of P. acidilactici UL5 (4 x 10(10) CFU/animal) on the propagation of Listeria monocytogenes LSD348 in intestine, liver and spleen was negligible. P acidilactici UL5 did not appear competitive with the mouse intestinal flora and was not detectable in fecal samples collected two days after administration. However, double-agar-layer activity assay showed the ability of P. acidilactici UL5 colonies recovered from fecal samples one day after administration to produce pediocin PA-1 and inhibit L monocytogenes. Moreover, repeated doses (250 mu g/day for three consecutive days) of purified pediocin PA-1 provided up to 2-log reductions in fecal listerial counts compared to the infected control group and slowed pathogen translocation into the liver and spleen, leading to the disappearance of L monocytogenes infection in these two organs within six days. Neither P. acidilactici UL5 nor ingested purified pediocin PA-1 had any negative effect on feed intake or body weight development. Pediocin PA-1 did not affect the composition of the mouse intestinal flora, suggesting a potential advantage over other inhibitory agents as a prophylactic measure against L monocytogenes. (C) 2009 Elsevier B.V. All rights reserved.
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