期刊
INTERNATIONAL JOURNAL OF FOOD MICROBIOLOGY
卷 129, 期 1, 页码 88-92出版社
ELSEVIER SCIENCE BV
DOI: 10.1016/j.ijfoodmicro.2008.11.001
关键词
Probiotics; Interpolymer complex; Bifidobacterium longum Bb-46; Supercritical CO(2)
资金
- Ellipsoid Technology (Pty) Ltd
- University of Pretoria
Gastric acidity is the main factor affecting viability of probiotics in the gastrointestinal tract (Grr). This study investigated the survival in simulated gastrointestinal fluids of Bifidobacterium longum Bb-46 encapsulated in interpolymer complexes formed in supercritical carbon dioxide (scCO(2)). Bacteria were exposed sequentially to simulated gastric fluid (SGF, pH 2) for 2 h and simulated intestinal fluid (SIF, pH 6.8) for 6 or 24 h. Total encapsulated bacteria were determined by suspending 1 g of product in SIF for 6 h at 37 degrees C prior to plating out. Plates were incubated anaerobically at 37 degrees C for 72 h. The interpolymer complex displayed pH-responsive release properties, with little to no release in SGF and substantial release in SIR There was a limited reduction in viable counts at the end of exposure period due to encapsulation. Protection efficiency of the interpolymer complex was improved by addition of glyceryl monostearate (GMS). Gelatine capsules delayed release of bacteria from the interpolymer complex thus minimizing time of exposure to the detrimental conditions. Use of poly(caprolactone) (PCL), ethylene oxide-propylene oxide triblock copolymer (PEO-PPO-PEO) decreased the protection efficiency of the matrix. Interpolymer complex encapsulation showed potential for protection of probiotics and therefore for application in food and pharmaceuticals. (C) 2008 Elsevier B.V. All rights reserved.
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