期刊
INTERNATIONAL JOURNAL OF EPIDEMIOLOGY
卷 37, 期 -, 页码 41-45出版社
OXFORD UNIV PRESS
DOI: 10.1093/ije/dym285
关键词
EBV; cell lines; Biobanks; genetic epidemiology
资金
- Medical Research Council [G0901461, G0100594] Funding Source: Medline
- Medical Research Council [G0100594, MC_qA137853] Funding Source: researchfish
- MRC [G0100594] Funding Source: UKRI
EBV immortalized B lymphocyte cell lines have been extensively used as a source of biological material for functional and molecular studies and represent a potentially limitless source of genomic DNA. Current technologies for EBV transformation are costly and use relatively large volumes of peripheral blood. Alternative methods were examined to determine whether smaller volumes of cryo-preserved whole blood could be subsequently transformed and which could provide a more cost-effective strategy for large population-based studies such as UK Biobank. A successful method was established where viable B cells were positively selected from 0.5 ml cryo-preserved whole blood samples. These were EBV transformed in microtitre plates and subsequently expanded in culture. A pilot study within UK Biobank was performed, which confirmed its potential usefulness for this study.
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