Resonance scattering spectrum detection of trace UO22+ using nanogold probe as catalyst of Cu(II)-glucose reaction

exhibited catalytic effect on the cracking reaction of double-stranded DNA (dsDNA) to form a short single-stranded DNA (ssDNA) that protected the nanogold (NG) to produce stable NGssDNA conjugate. The un-protected NG was aggregated to form NG aggregates (NGA) that appeared a resonance scattering (RS) peak at 542nm. Unlike NGA, the NGssDNA exhibited a strong catalytic activity on the Cu2O particle reaction of Fehling reagent-glucose that can be monitored by RS technique at 608nm. When the concentration increased, the NGssDNA increased, and the RS intensity at 608nm increased. The increased RS intensity I 608nm was linear to the concentration in the range of 15200pmolL1, with a regression equation of I 608nm=1.24C+4.6, and a detection limit of 5pmolL1. This new RS assay was applied to assay in water sample, with satisfactory results.