期刊
INTERNATIONAL JOURNAL OF DEVELOPMENTAL BIOLOGY
卷 53, 期 8-10, 页码 1445-1455出版社
UNIV BASQUE COUNTRY UPV-EHU PRESS
DOI: 10.1387/ijdb.072417cl
关键词
cardiogenesis; chick embryo; fate map; gastrulation; precardiac cell
资金
- Spanish Ministerio de Educacion y Ciencia [BFU2007-66350/BFI]
- Consejeria de Economia [3PR05A058 PRI07A005]
- Comercio e Innovacion of the Junta de Extremadura
Fate maps are required to address questions about the commitment and differentiation of precardiac cells. Here, we report a detailed study of the precardiac cells located at the level of the primitive streak, employing different experiments with a variety of techniques combining double transplantations, microinjections and immunocytochemistry. Most cells of the more rostral segments of the primitive streak were found to contribute cells to the endodermal layer, adjacent to precardiac mesodermal cells of the heart forming region whose provenance was in the immediately more caudal segments of the primitive streak. We established a close spatiotemporal relationship between the two cell layers and the expression of their specific cardiac markers (cNkx-2.5, Bmp2, Cripto, Usmaar, dHand, GATA4, Pitx2, Hex, Fgf8, AMHC1 and VMHC1). We also analyzed the ability of precardiac cells to differentiate when they are transplanted to ectopic locations or are subjectedtothe influence of the organizer. We propose that the precardiac cells of the primitive streak form at least two groups with different significance. One, regulated by mediation of the organizer, is located preferentially in the more rostral region of the primitive streak. It consists of the prospective cells of the endoderm layer, with a hierarchic pattern of expression of different genes characterized by its capacity for induction and regulation of a second group of cells. This second group is located preferentially in the more caudal segments, and is fated to form the precardiac mesoderm, whose differentiation would be characterized by the expression of various specific genes.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据