期刊
METABOLIC ENGINEERING
卷 31, 期 -, 页码 163-170出版社
ACADEMIC PRESS INC ELSEVIER SCIENCE
DOI: 10.1016/j.ymben.2015.08.002
关键词
Cyanobacteria; Biofuel; 3-Hydroxypropionate; Malonate semialdehyde
资金
- Kaiteki Institute
- National Science Foundation [MCB-1139318]
- National Science Foundation under the American Recovery and Reinvestment Act (ARRA)
- Direct For Biological Sciences [1139318] Funding Source: National Science Foundation
- Div Of Molecular and Cellular Bioscience [1139318] Funding Source: National Science Foundation
Photosynthetic conversion of CO2 to chemicals using cyanobacteria is an attractive approach for direct recycling of CO2 to useful products. 3-Hydroxypropionic acid (3HP) is a valuable chemical for the synthesis of polymers and serves as a precursor to many other chemicals such as acrylic acid. 3HP is naturally produced through glycerol metabolism. However, cyanobacteria do not possess pathways for synthesizing glycerol and converting glycerol to 3HP. Furthermore, the latter pathway requires coenzyme B12, or an oxygen sensitive, coenzyme B12-independent enzyme. These characteristics present major challenges for production of 3HP using cyanobacteria. To overcome such difficulties, we constructed two alternative pathways in Synechococcus elongatus FCC 7942: a malonyl-CoA dependent pathway and a p-alanine dependent pathway. Expression of the malonyl-CoA dependent pathway genes (malonyl-CoA reductase and malonate semialdehyde reductase) enabled S. elongatus to synthesize 3HP to a final titer of 665 mg/L beta-Alanine dependent pathway expressing S. elongatus produced 3HP to final titer of 186 mg/L. These results demonstrated the feasibility of converting CO2 into 3HP using cyanobacteria. (C) 2015 International Metabolic Engineering Society. Published by Elsevier Inc. All rights reserved.
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