期刊
METABOLIC ENGINEERING
卷 31, 期 -, 页码 35-43出版社
ACADEMIC PRESS INC ELSEVIER SCIENCE
DOI: 10.1016/j.ymben.2015.06.008
关键词
Metabolite biosensor; Synthetic biology; Metabolic engineering; Pathway optimization; Dynamic pathway regulation
资金
- National Science Foundation [MCB1453147, MCB1331194]
- Defense Advanced Research Projects Agency [D13A000038]
- International Center for Advanced Renewable Energy and Sustainability (I-CARES)
- Div Of Molecular and Cellular Bioscience
- Direct For Biological Sciences [1453147] Funding Source: National Science Foundation
Quantification and regulation of pathway metabolites is crucial for optimization of microbial production bioprocesses. Genetically encoded biosensors provide the means to couple metabolite sensing to several outputs invaluable for metabolic engineering. These include semi-quantification of metabolite concentrations to screen or select strains with desirable metabolite characteristics, and construction of dynamic metabolite-regulated pathways to enhance production. Taking inspiration from naturally occurring systems, biosensor functions are based on highly diverse mechanisms including metabolite responsive transcription factors, two component systems, cellular stress responses, regulatory RNAs, and protein activities. We review recent developments in biosensors in each of these mechanistic classes, with considerations towards how these sensors are engineered, how new sensing mechanisms have led to improved function, and the advantages and disadvantages of each of these sensing mechanisms in relevant applications. We particularly highlight recent examples directly using biosensors to improve microbial production, and the great potential for biosensors to further inform metabolic engineering practices. (C) 2015 International Metabolic Engineering Society. Published by Elsevier Inc. All rights reserved.
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