期刊
INTERNATIONAL JOURNAL OF CANCER
卷 131, 期 4, 页码 E449-E462出版社
WILEY-BLACKWELL
DOI: 10.1002/ijc.27353
关键词
V?9Vd2 T cells; immunotherapy; ovarian carcinoma; ascites; prostaglandin E2
类别
资金
- INCA PL [2008-034]
- Canceropole Grand Ouest
- Comite de la Recherche Clinique du CHU de Rennes
Epithelial ovarian cancer (EOC) usually spreads into the peritoneal cavity, thereby providing an opportunity for intraperitoneal adoptive immunotherapy with V?9Vd2 T lymphocytes, a T cell subpopulation endowed with high lytic properties against tumor cells. However, previous studies have reported that V?9Vd2 T cells fail to expand from peripheral blood mononuclear cells in one-third of patients with cancer. Here, from a cohort of 37 patients with EOC, a multiple correspondence analysis identified three populations, one of which was not suitable for V?9Vd2 T-cell adoptive therapy. Interestingly, the ineligible patients were identified based on the frequency of V?9Vd2 T cells in their peripheral blood and the patients' age. The average time to tumor recurrence was also found to be significantly different between the three populations, suggesting that the innate immune response is involved in EOC prognosis. A dramatic decrease in the lytic properties of V?9Vd2 T cells occurred following incubation with ascitic supernatant and was found to be associated with reduced perforin/granzyme degranulation. Prostaglandin E2, but not IL-6, IL-10, VEGF or TGF-beta, showed immunosuppressive effects in V?9Vd2 T cells. Interestingly, our results emphasize that pretreating ovarian tumor cells with zoledronate partially reverses the immunosuppressive effects of ovarian cancer-associated ascites and restores a high level of lytic activity. These data sustain that optimal V?9Vd2 T-cell adoptive immunotherapy previously requires counteracting the tumor immunosuppressive microenvironment. Altogether, our findings provide a rationale for clinically evaluating V?9Vd2 T-cell adoptive immunotherapy with intraperitoneal carcinomatosis presensitization by zoledronate in patients with EOC.
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