期刊
INTERNATIONAL JOURNAL OF CANCER
卷 123, 期 9, 页码 2105-2112出版社
WILEY-LISS
DOI: 10.1002/ijc.23685
关键词
episome; real-time QPCR; Epstein-Barr virus; nasopharyngeal carcinoma; epithelial cells
类别
资金
- The Ohio State University Comprehensive Cancer Center
- NCI [CA 16058]
- Gilbert and Kathryn Mitchell Endowment [CA109232, DE018304]
Epstein-Barr virus (EBV) is the prototypical example for episomal persistence of genetic information. Yet, little is known about how this viral episome is lost. Episome loss occurs naturally in nasopharyngeal carcinoma (NPC) upon explantation into culture. Using whole-genome profiling, we found evidence for 2 different pathways of episome loss: (i) rapid loss of the entire episome or (ii) successive mutation/deletion of the episome until at least 1 essential cis-element is destroyed. This second phenotype was seen in a clone of HONE-1 NPC cells that maintains the EBV episome for prolonged time in culture. The conceptual insights provided by our quantitative analysis should aid our understanding of mammalian episomes, as well as lead to designs to cure latent viral infection. (c) 2008 Wiley-Liss, Inc.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据