期刊
INTERNATIONAL JOURNAL OF BIOLOGICAL SCIENCES
卷 8, 期 10, 页码 1408-1417出版社
IVYSPRING INT PUBL
DOI: 10.7150/ijbs.4597
关键词
miR-145; IRS1; inhibit; dedifferentiated fat cells; adipogenesis
资金
- National High Technology Research and Development Program of China (863 Program) [2011AA100304]
- Natural Science Foundation of Guangdong [S2011010001380]
- Key Projects in the Science & Technology Pillar Program of Guangzhou [2008Z1-E121]
Generally, most miRNAs that were up-regulated during differentiation promoted adipogenesis, but our research indicated that up-regulation of miR-145 in porcine preadipocytes did not promote but inhibit adipogenesis. In this study, miR-145 was significantly up-regulated during porcine dedifferentiated fat (DFAT) cells differentiation. In miR-145 overexpressed DFAT cells, adipogenesis was inhibited and triglycerides accumulation was decreased after hormone stimulation (P<0.05). Furthermore, up-regulation of miR-145 expression repressed induction of mRNA levels of adipogenic markers, such as CCAAT/enhancer-binding protein a (C/EBP alpha), and peroxisome proliferator-activated receptor gamma 2 (PPAR gamma 2). These effects caused by miR-145 overexpression were mediated by Insulin receptor substrate 1 (IRS1) as a mechanism. These data suggested that induced miR-145 expression during differentiation could inhibit adipogenesis by targeting IRS1, and miR-145 may be novel agent for adipose tissue engineering.
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