期刊
INTERNATIONAL JOURNAL OF BIOLOGICAL SCIENCES
卷 8, 期 4, 页码 498-511出版社
IVYSPRING INT PUBL
DOI: 10.7150/ijbs.3723
关键词
bovine; fetal fibroblasts; iPS cells; defined-factor fusion proteins
资金
- National Natural Science Foundation of China [30800784]
- National Basic Research Program [2009CB941004]
- National High-Tech RD Program [2008AA101003, 2008AA101010, 2011AA100307]
- National Transgenic Breeding Program [2009ZX08008-007B]
Pluripotent stem cells from domesticated animals have potential applications in transgenic breeding. Here, we describe induced pluripotent stem (iPS) cells derived from bovine fetal fibroblasts by lentiviral transduction of Oct4, Sox2, Klf4 and c-Myc defined-factor fusion proteins. Bovine iPS cells showed typical colony morphology, normal karyotypes, stained positively for alkaline phosphatase (AP) and expressed Oct4, Nanog and SSEA1. The CpG in the promoter regions of Oct4 and Nanog were highly unmethylated in bovine iPS cells compared to the fibroblasts. The cells were able to differentiate into cell types of all three germ layers in vitro and in vivo. In addition, these cells were induced into female germ cells under defined culture conditions and expressed early and late female germ cell-specific genes Vasa, Dazl, Gdf9, Nobox, Zp2, and Zp3. Our data suggest that bovine iPS cells were generated from bovine fetal fibroblasts with defined-factor fusion proteins mediated by lentivirus and have potential applications in bovine transgenic breeding and gene-modified animals.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据