期刊
INTERNATIONAL JOURNAL OF BIOLOGICAL SCIENCES
卷 8, 期 4, 页码 451-458出版社
IVYSPRING INT PUBL
DOI: 10.7150/ijbs.4038
关键词
RECK; hepatocellular carcinoma; methylation
资金
- Natural Science Foundation of China [30901722, 81000970, 81030041, 31171321, 81101622]
- Shanghai Science and Technology Committee [11ZR1449500, 10411963100, 10ZR1439900, 10ZR1439600]
- Key Basic Research Project of China [2010CB945600, 2011CB966200]
- NSFC, China [30921006]
- Natural Science Foundation of Jiangsu [BK2011251]
- Health Talents and 333 Project for Jiangsu [LJ201157, RC2011038, BRA2011038]
- Technology Planning Project of Changzhou [CS2008214, CS20102021, CS20102022]
- Talents Project (831 and health) of Changzhou
Background: To evaluate the promoter methylation status of RECK gene and mRNA expression in patients with hepatocellular carcinoma (HCC). Methods: We analyzed RECK methylation by MSP, and RECK mRNA by real-time PCR in 74 HCC. The liver cell lines (7721, Chang and Hep-G2) were treated with 5-Aza-CdR and TSA. Results: RECK mRNA were lower in HCC tissues (Mean (-Delta Ct) = -3.29) than that in Non-Hcc tissues (Mean (-Delta Ct) = -2.42). Expression of RECK was elevated in only 24 (32.43%) of the 74 HCC patients but decreased (-Delta Delta Ct<0) in 50 (67.57%) of the patients. RECK promoter was hypermethylated in 55.4% (41/74) of HCCs, and in only 17.6% (13/74) of Non-Hcc samples. RECK mRNA were lower in HCC patients with hypermethylation (Delta MI>=0.5) (Mean (-Delta Delta Ct) = -1.75) than those with demethylation (Delta MI<0.5) (Mean (-Delta Delta Ct) = 0.05), and there is a decreased tendency for RECK mRNA in HCC patients with promoter hypermethylation (p = 0.002). There was a significantly correlation found between RECK mRNA and poor survival after surgery. After treated by 5-Aza-CdR and TSA, we found that RECK mRNA induced different changes in 7721, Chang and Hep-G2 cells. And RECK demethylation also induced by epigenetic inhibitors. Conclusion: The results suggested that the hypermethylation may lead to promoter silencing of RECK mRNA and associated with poor survival in HCC.
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