期刊
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
卷 120, 期 -, 页码 2242-2246出版社
ELSEVIER
DOI: 10.1016/j.ijbiomac.2018.08.054
关键词
Eriocheir japonica sinensis; Osmoregulation; Gill; qPCR; Salinity change
资金
- National Natural Science Foundation of China [41301050]
- Natural Science Foundation of Jiangsu Province [BK20171276, 12KJA180009]
- Foundation of Jiangsu Provincial Key Laboratory [JKLBS2016001]
- Qing Lan Project
- 333 Project
- Outstanding Young Talents of YCTU
Desalination of marine species has become an important development direction for aquaculture in China and other countries. However, that how to regulate the salt balance to adapt to new freshwater habitats is a serious challenge for marine species in desalination of aquaculture. In the study, Chinese mitten crabs (Eriocheir japonica sinensis) was selected to analyse the adaptively differential expression in salinity changes for their novel characteristics of life history. The results showed that gill was the most relevant tissue in osmoregulation that was validated by biomarkers (Na+ /K+-ATP, V-type H+-ATPase) with qPCR. Na+ /K+-ATPase is a primary transporter and maintains the body fluid osmolality by actively pumping Na+ to the hemolymph, and V-type Fl(+)-ATPase is responsible for acid-base balance and nitrogen excretion. So both transcriptome data and qPCR results showed the significantly differential expression of Na+ /K+-ATPase and V-type H-ATPase in gills. Moreover, NAK-alpha had the most significantly differential expression level in salinity change, and other genes such as GST, HSP90, S27, UBE, VATS also revealed significantly up-regulation. They are considered the key enzymes during the transition from a marine environment to land. Present results have provided a foundation to further understand the molecular adaptive mechanism in desalination of marine species. (C) 2018 Published by Elsevier B.V.
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