期刊
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
卷 120, 期 -, 页码 2589-2596出版社
ELSEVIER SCIENCE BV
DOI: 10.1016/j.ijbiomac.2018.09.035
关键词
Lotus leaf flavonoids (LLF); Pancreatic alpha-amylase; Inhibitory
资金
- National Science-technology Support Plan Projects [2014BAD04B01]
In this study, lotus leaf flavonoids (LLF) were found to show a notable inhibition activity on alpha-amylase in a mixed-type manner with IC50 value of (5.58 +/- 0.10) mg/mL. The intrinsic fluorescence of alpha-amylase was quenched by the interaction with LLF through a static quenching mechanism, and LLF-alpha-amylase complex was spontaneously formed mainly driven by the hydrophobic interaction and hydrogen bonding. Multispectroscopic analyses (synchronous fluorescence, three-dimensional fluorescence, circular dichroism (CD) and fourier transform infrared spectra (FT-IR)) comprehensively demonstrated that the binding of LLF to alpha-amylase would change the conformation and microenvironment of alpha-amylase, resulting in inhibiting the enzyme activity. The present study indicated that LLF had potential to be as an ingredient in functional food for the prevention of type-2 diabetes. (C) 2018 Elsevier B.V. All rights reserved.
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