期刊
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
卷 71, 期 -, 页码 59-67出版社
ELSEVIER SCIENCE BV
DOI: 10.1016/j.ijbiomac.2014.04.054
关键词
Burkholderia sacchari; Poly(3-hydroxybutyrate-co-4-hydroxybutyrate); Wheat straw lignocellulosic hydrolysates
资金
- European Union 7th Framework Programme (FP7) [246449]
- Fundacao para a Ciencia e Tecnologia, Portugal [SFRH/BPD/68587/2010, SFRH/BPD/26678/2006]
- Fundação para a Ciência e a Tecnologia [SFRH/BPD/26678/2006] Funding Source: FCT
Burkholderia sacchari DSM 17165 is able to grow and produce poly(3-hydroxybutyrate) both on hexoses and pentoses. In a previous study, wheat straw lignocellulosic hydrolysates (WSH) containing high C6 and C5 sugar concentrations were shown to be excellent carbon sources for P(3HB) production. Using a similar feeding strategy developed for P(3HB) production based on WSH, fed-batch cultures were developed aiming at the production of the copolymer P(3HB-co-4HB) (poly(3-hydroxybutyrate-co-4-hydroxybutyrate)) by B. sacchari. The ability of this strain to synthesize P(3HB-co-4HB) was first shown in shake flasks using gamma-butyrolactone (GBL) as precursor of the 4HB units. Fed-batch cultures using glucose as carbon source (control) and GBL were developed to achieve high copolymer productivities and 4HB incorporations. The attained P(3HB-co-4HB) productivity and 4HB molar% were 0.7 g/(L h) and 4.7 molar%, respectively. The 4HB incorporation was improved to 6.3 and 11.8 molar% by addition of 2 g/L propionic and acetic acid, respectively. When WSH were used as carbon source under the same feeding conditions, the values achieved were 0.5 g/(L h) and 5.0 molar%, respectively. Burkholderia sacchari, a strain able to produce biopolymers based on xylose-rich lignocellulosic hydrolysates, is for the first time reported to produce P(3HB-co-4HB) using gamma butyrolactone as precursor. (C) 2014 Elsevier B.V. All rights reserved.
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