期刊
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
卷 55, 期 -, 页码 282-288出版社
ELSEVIER SCIENCE BV
DOI: 10.1016/j.ijbiomac.2013.01.025
关键词
Gleditsia sinensis gum; Galactomannan; Hydrolysis; Oligosaccharide; beta-Mannanase
资金
- Specific Programs in Graduate Science and Technology Innovation of Beijing Forestry University [BLYJ201111]
- National Natural Science Foundation of China [31270624]
- Beijing Natural Science Foundation [6122023]
Enzymatic hydrolysis of Gleditsia sinensis gum was performed to produce manno-oligosaccharides having functional applications as dietary fiber and prebiotics. The optimum hydrolysis conditions, including enzyme loading, temperature and time, from response surface methodology were 8.1 U/g, 57.4 degrees C and 34.1 h, respectively. The yield of DP 1-5 oligosaccharides was 75.9% (29.1 g/L). The Michaelis-Menten kinetics and molecular weight distribution were determined. The obtained oligosaccharides were further separated by HPLC and SEC, and the galactose distribution of G. sinensis gum was elucidated. Results indicated that G. sinensis gum has potential to produce value-added oligosaccharides in food industries. (C) 2013 Elsevier B.V. All rights reserved.
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