期刊
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
卷 62, 期 -, 页码 348-351出版社
ELSEVIER SCIENCE BV
DOI: 10.1016/j.ijbiomac.2013.09.042
关键词
Chitooligosaccharides; Hydrogen peroxide; Antibacterial activity
In this study, chitooligosaccharides were prepared from cicada slough of Cryptotympana atrata Fabricius by hydrolysis using hydrogen peroxide (H2O2). Factors affecting the hydrolysis of chitosan were investigated and the optimum hydrolysis conditions were as follows: time, 4 h; temperature, 65 degrees C; amount of H2O2, 2% (v/v); and pH, 5. Under these conditions, the average degree of polymerisation decreased to similar to 4.5. The Fourier transform infrared spectra and product sugar composition indicate that there were no significant chemical changes in the backbones of the chitosan treated with H2O2. The chitooligosaccharides had high antibacterial activity against Bacillus subtilis, Staphylococcus aureus, and Escherichia coli at the concentration of 100 mg/mL. (C) 2013 Elsevier B.V. All rights reserved.
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