期刊
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
卷 62, 期 -, 页码 321-329出版社
ELSEVIER SCIENCE BV
DOI: 10.1016/j.ijbiomac.2013.09.032
关键词
Chitosan; Diabetes mellitus; Gallic acid; Graft copolymer; Redox pair
资金
- National Natural Science Foundation of China [31101216]
- Natural Science Foundation of the Education Committee of Jiangsu province [11KJB550006]
- Priority Academic Program Development of Jiangsu Higher Education Institutions
In this study, chitosan grafted copolymer with gallic acid (GA) was synthesized by a novel and efficient free radical mediated method. The optimal grafting conditions, structural characterization, a-glucosidase and a-amylase inhibitory activities of chitosan grafted copolymers were investigated. Results showed that the maximum grafting ratio (128.3 mg GA equivalents/g) was obtained at 12 h with 5 g/L chitosan, 16 g/L GA, 2 g/L ascorbic acid and 0.2 M hydrogen peroxide. UV-vis, Fourier-transform infrared and nuclear magnetic resonance spectroscopy all confirmed the successful grafting of GA onto chitosan. The conjugation of GA onto chitosan probably occurred between amine (C-2), hydroxyl groups (C-3 and C-6) of chitosan and carboxyl groups of GA, forming amide and ester linkages, respectively. Differential scanning calorimetry and X-ray diffraction spectra indicated that GA grafted chitosan (GA-g-chitosan) had decreased thermal stability and crystallinity as compared to chitosan. Notably, GA-g-chitosan showed increased alpha-glucosidase and alpha-amylase inhibitory activity with the increase of grafting ratio. These results indicated the potential of GA-g-chitosan in the development of an effective anti-diabetic agent. (C) 2013 Elsevier B.V. All rights reserved.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据