期刊
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
卷 62, 期 -, 页码 336-340出版社
ELSEVIER
DOI: 10.1016/j.ijbiomac.2013.09.007
关键词
Sepioteuthis lessoniana; Sulfated chitosan; Structural analysis; Antioxidant activity; Anticoagulant activity
资金
- Centre for Marine Living Resources and Ecology (CMLRE), Ministry of Earth Sciences, Cochin
beta-Chitosan extracted from the gladius of Sepioteuthis lessoniana was sulfated with chlorosufonic acid. The structural features of sulfated chitosan were analyzed by NMR spectroscopy. The elemental composition was analyzed using elemental analyzer. The molecular weight of sulfated chitosan estimated through viscometry was calculated as 12.47 x 10(4) Da. The water solubility of sulfated chitosan was found to be 81%. The sulfated chitosan was able to scavenge the DPPH, superoxide and hydroxyl radicals and the inhibitory concentration (IC50) of those free radicals was found to be 4, 0.428 and 0.473 mg/ml respectively. Sulfated chitosan also showed reducing and chelating property. The IC50 value of reducing and chelating ability was found to be 0.316 and 5.64 mg/ml respectively. The anticoagulant activity was determined for human plasma with respect to activated partial thromboplastin time and prothrombin time and it was 7.13 IU and 1.26 IU, respectively. (C) 2013 Elsevier B.V. All rights reserved.
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