期刊
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
卷 61, 期 -, 页码 347-352出版社
ELSEVIER
DOI: 10.1016/j.ijbiomac.2013.07.013
关键词
Astragalus polysaccharide; Lipopolysaccharide; Inflammatory response
资金
- Natural Science Foundation of China [31001017, 31272464]
- Program for New Century Excellent Talents [NCET-12-0476]
- National Key Technology R&D Program of the Ministry of Science and Technology [2013BAD10B02]
- Science & Technological Project of Shaanxi Province, China [2011KTCQ02-02, 2011KTDZ02-01-01]
- Program for Shaanxi Youth Scientific Talents [2012KJXX-18]
As the major constituent of Radix Astragali, Astragalus polysaccharide (APS) is known for its anti-inflammation and immunomodulatory functions. The objective of this study was to investigate the effect of APS on inflammatory response and structural changes in lipopolysaccharide (LPS)-infected Caco2 cells. Caco2 cells were co-cultured with APS and LPS, with APS added after the addition of LPS (post-addition), before the addition of LPS (pre-addition), or simultaneously with the addition of LPS (simultaneous addition). The mRNA expression of inflammatory indicators and tight junctions was measured by RT-qPCR. Short circuit current (Isc) was recorded by an Ussing chamber system. Addition of APS significantly down-regulated the expression of TNF-alpha, IL-1 beta and IL-8 (P<0.05) and the Isc levels (P<0.05) of LPS-infected Caco2 cells for all three administration treatments. The minimum anti-inflammatory concentration of APS was 50, 100, and 100 mu g/mL for pre-, post-, and simultaneous additions of APS, respectively. The mRNA expression of zonula occludens-1 (ZO-1) and ocdudin was significantly up-regulated for post- and pre-additions of APS, respectively (P<0.05). Results suggested that APS had anti-inflammatory and structure protective properties for LPS-infected Caco2 cells, and may be used as a preventative treatment for intestine cells. (C) 2013 Elsevier B.V. All rights reserved.
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