期刊
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
卷 51, 期 1-2, 页码 97-104出版社
ELSEVIER SCIENCE BV
DOI: 10.1016/j.ijbiomac.2012.04.021
关键词
Bovine serum albumin; Alkaline-induced intermediate; Polyethylene glycols; CD spectra; Fluorescence spectroscopy
资金
- National Natural Science Foundation of China [21105063, 21175091]
- Science and Technology Planning Project of He'nan Province, China [112102210416]
- Foundation of He'nan Educational Committee [2008B150017, 200410483002]
In the present study, the formation of one molten globule-like unfolding intermediate of bovine serum albumin (BSA) at pH 11.2 has been established with the help of circular dichroism (CD) spectra, fluorescence spectroscopy and 'phase diagram' approach. Additionally, we have shown the conformational changes occurring in the pH 11.2 intermediate of BSA when it was exposed to different molecular weight polyethylene glycols (PEGs) at varying concentrations. When the pH 11.2 intermediate of BSA was treated by PEG 400 there was induction of secondary and non-native tertiary contacts. In case of PEG 8000 and PEG 20,000, the loss in secondary as well as tertiary structure was observed. PEG 8000 and 20,000 altered the conformation of the pH 11.2 intermediate and resulted in its transition to another intermediate state in which the hydrophobic patches were inaccessible. (c) 2012 Elsevier B.V. All rights reserved.
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