期刊
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
卷 51, 期 4, 页码 406-411出版社
ELSEVIER
DOI: 10.1016/j.ijbiomac.2012.06.012
关键词
Chlorambucil; FTIR spectroscopy; UV-visible spectroscopy; Circular dichroism spectroscopy; Molecular docking
资金
- Department of Biotechnology [DBT-JRF/08-09/150]
- University Grants Commission (UGC), Government of India
- Indian Council of Medical
Chlorambucil (CMB) is an anticancer drug used for the treatment of variety of cancers. Structural and conformational changes associated with DNA after binding with CMB were explored using spectroscopic techniques to get insight into the mechanism of action of CMB at molecular level. Different molar ratios of CMB-DNA complex were prepared with constant DNA concentration under physiological conditions. FTIR spectroscopy, UV-visible spectroscopy, CD spectroscopy and molecular docking studies were employed to determine the binding site and binding constant of CMB with DNA. The results show CMB binds DNA through nitrogenous bases (thymine, guanine and cytosine). The binding constant was calculated to be 1.3 x 10(3) M-1, which suggests weak binding of CMB with DNA double helix. FTIR and CD results show that CMB do not disturb native B-conformation of DNA and it continues to remain in its B conformation even at higher concentrations of CMB. The molecular docking results are in corroboration with our experimental results and provides structural insight into the interaction site. (C) 2012 Elsevier B.V. All rights reserved.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据