期刊
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
卷 50, 期 1, 页码 219-229出版社
ELSEVIER
DOI: 10.1016/j.ijbiomac.2011.10.026
关键词
Alkaline alpha-amylase; Bacillus; Detergent compatibility; Response surface method; Submerged fermentation
资金
- Council of Scientific and Industrial Research (CSIR), New Delhi
- UGC-SAP
- Department of MBBT
- UGC, New Delhi
- DBT, New Delhi
A strain AS-S01a, capable of producing high-titer alkaline alpha-amylase, was isolated from a soil sample of Assam, India and was taxonomically identified as Bacillus subtilis strain AS-S01a. Optimized alpha-amylase yield by response surface method (RSM) was obtained as 799.0 U with a specific activity of 201.0 U/mg in a process control bioreactor. A 21.0 kDa alkaline alpha-amylase purified from this strain showed optimum activity at 55 degrees C and pH 9.0, and it produced high molecular weight oligosaccharides including small amount of glucose from starch as the end product. The K-m and V-max values for this enzyme towards starch were determined as 1.9 mg/ml and 198.21 mu mol/min/mg, respectively. The purified alpha-amylase retained its activity in presence of oxidant, surfactants, EDTA and various commercial laundry detergents, thus advocating its suitability for various industrial applications. (C) 2011 Elsevier B.V. All rights reserved.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据