期刊
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
卷 49, 期 4, 页码 466-470出版社
ELSEVIER
DOI: 10.1016/j.ijbiomac.2011.05.026
关键词
Polysaccharides; Extracting condition; Antioxidant activity; Plantago asiatica
The extraction conditions of polysaccharides from Plantago asiatica L seeds were investigated. Four parameters affecting the polysaccharides extraction, extraction times, water to sample, extraction temperature and single extraction time, were determined by orthogonal experiments. Under the optimized conditions, the polysaccharides yield of P. asiatica L seeds was 2.467%. The antioxidant activities of the polysaccharides were investigated. The reducing power of the polysaccharides was dose dependent, and the reducing capacity of the polysaccharides was inferior to butylated hydroxytoluene, which is known to be a strong reducing agent. The scavenging rates of the polysaccharides on superoxide and 1,1-diphenyl-2-picrylhydrazyl radicals were79.7% and 81.4%, at polysaccharides concentration of 0.75 mg/mL, respectively, a scavenging rates approximately similar to that of 0.75 mg/mL ascorbic acid (83.5% and 85.1%, respectively). Furthermore, it exhibited a moderate concentration-dependent ABTS radical scavenging activity, ferrous ion chelating potency and H2O2 scavenging activity. The data obtained in the in vitro models clearly establish the antioxidant potency of the polysaccharides extracted from Semen Plan taginis. (C) 2011 Elsevier B.V. All rights reserved.
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