期刊
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
卷 49, 期 4, 页码 674-680出版社
ELSEVIER
DOI: 10.1016/j.ijbiomac.2011.06.027
关键词
Urease; Urea; Canavalia ensiformis; Cajanus cajan; Gold nanoparticles; Immobilization
资金
- Council of Scientific and Industrial Research (CSIR), New Delhi
- Department of Science and Technology (DST, UNANST)
Ureases isolated from leguminous sources, Canavalia ensiformis and Cajanus cajan were immobilized onto gold nanoparticles (nano-ureases). Optimization of the urease immobilization was carried using response surface methodology based on Central Composite Design. Immobilization efficiency of nano-urease from C. ensiformis and C cajan were found to be 215.10% and 255.92%, respectively. The methodology adopted has deviation of 2.56% and 3.01% with respect to experimental values in case of C. ensiformis and C. cajan, respectively. Nano-urease from C. cajan has broad physico-chemical parameters with pH optimum from 7.1 to 7.3 and temperature optimum from 50 to 70 degrees C. Nano-urease from C. ensiformis has sharp pH and temperature optima at 7.3 and 70 degrees C. respectively. Fourier transform infra-red spectroscopy has revealed involvement of groups viz, amino, glycosyl moiety, etc. in urease immobilization onto gold nano-particles. Transmission and scanning electron micrographs revealed that arrangement of urease onto gold nanoparticles from C. ensiformis was uniform while it was localized in case of C. cajan. Nano-urease from C. ensiformis has higher specificity and catalysis toward urea as compared to nano-urease from C. cajan. Nano-ureases from both sources are equally stable for 6 months under dried conditions and can be used for 10 washes. (C) 2011 Elsevier B.V. All rights reserved.
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