期刊
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
卷 49, 期 5, 页码 1124-1130出版社
ELSEVIER
DOI: 10.1016/j.ijbiomac.2011.09.011
关键词
Auricularia auricula; Polysaccharide; Carboxymethylation; Characterization; Antioxidant activity
The chemical carboxymethylated polysaccharide (CMAAP), which is derived from the poorly water-soluble, crude polysaccharide from Auricularia auricula (AAP), was prepared. The degree of substitution of CMAAP was 0.857, and its solubility in water reached 0.6 mg/mL, much higher than that of AAP (0.1 mg/mL). One fraction, CMAAP22, was purified. Chemical analysis indicated that CMAAP22 was composed of mannose and glucose in a ratio of 1.06:1 with a molecular weight of 3.4 x 10(6) Da. IR spectra and C-13 NMR spectra indicated that the C2. C4 and C6 positions were partially substituted by -CH2COOH. AFM revealed that CMAAP22 was spherical particle structure with a diameter of 80-300 nm. Moreover, the antioxidant activity of CMAAP22 was improved, nearly twice as much as that of the AAP, especially in the scavenging of DPPH and ABTS+. (C) 2011 Elsevier B.V. All rights reserved.
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