期刊
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
卷 46, 期 2, 页码 206-211出版社
ELSEVIER SCIENCE BV
DOI: 10.1016/j.ijbiomac.2009.12.006
关键词
Sheath; Glycoconjugate; Leptothrix cholodnii
资金
- Grants-in-Aid for Scientific Research [21370047] Funding Source: KAKEN
The sheath of Leptothrix cholodnii is constructed from a structural glycoconjugate, a straight-chained amphoteric heteropolysaccharide modified with glycine and cysteine. Though the structure of the glycan core is already determined, its modifications with amino acids and other molecules are not fully resolved In this study, we aimed to determine the chemical structure of the glycoconjugate as a whole Enantiomeric determination of cysteine in the sheath was performed and as a result. L-cysteine was detected. NMR spectroscopy was endeavored to determine overall structure of the glycoconjugate. Prior to NMR analysis, solubilization of the glycoconjugate was attempted by adding denaturing reagents or by derivatization As far as tested, sulfonation by performic acid oxidation was suitable for solubilization, but further improvement was achieved by N-acetylation. The approximate molecular weight of the derivative was estimated to be 4.5 x 10(4) by size-exclusion chromatography The NMR studies for the sulfonated glycoconjugate and its N-acetylated derivative revealed that the sheath glycoconjugate is a glycosaminoglycan consisting of a pentasaccharide repeating unit which is substoichiometrically esterified with 3-hydroxypropionic acid and stoichiometrically amidated with acetic acid and glycyl-L-cysteine. (C) 2009 Elsevier B V All rights reserved.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据