期刊
INTERNATIONAL JOURNAL OF BIOCHEMISTRY & CELL BIOLOGY
卷 44, 期 11, 页码 2051-2059出版社
PERGAMON-ELSEVIER SCIENCE LTD
DOI: 10.1016/j.biocel.2012.08.005
关键词
CAFs; miRNA; Breast cancer; Tumor microenvironment
资金
- National Natural Science Foundation of China [NSFC 81072147, NSFC 30940096]
- Natural Science Foundation of Chongqing Science & Technology Commission, China [CSTC2010BB5099]
- Major Program of Chongqing Medical University [XBZD201006]
- Doctoral Fund of Ministry of Education, China [20105503110001]
- Scientific Research Foundation for the Returned Overseas Scholars of Chongqing Medical University
Cancer-associated fibroblasts (CAFs) promote tumorigenesis, growth, invasion and metastasis of cancer, whereas normal fibroblasts (NFs) are thought to suppress tumor progression. Little is known about miRNAs expression differences between CAFs and NFs or the patient-to-patient variability in miRNAs expression in breast cancer. We established primary cultures of CAFs and paired NFs from six resected breast tumor tissues that had not previously received radiotherapy or chemotherapy treatment and analyzed with miRNAs microarrays. The array data were analyzed using paired SAM t-test and filtered according to alpha and q values. Pathway analysis was conducted using DAVID v6.7. We identified 11 dysregulated miRNAs in CAFs: three were up-regulated (miR-221-5p, miR-31-3p, miR-221-3p), while eight were down-regulated (miR-205, miR-200b, miR-200c, miR-141, miR-101, miR-342-3p, let-7g, miR-26b). Their target genes are known to affect cell differentiation, adhesion, migration, proliferation, secretion and cell-cell interaction. By our knowledge it is firstly identify the expression profiles of miRNAs between CAFs and NFs and revealed their regulation on the associated signaling pathways. (C) 2012 Elsevier Ltd. All rights reserved.
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