期刊
INTERNATIONAL JOURNAL OF BIOCHEMISTRY & CELL BIOLOGY
卷 44, 期 12, 页码 2095-2105出版社
PERGAMON-ELSEVIER SCIENCE LTD
DOI: 10.1016/j.biocel.2012.09.001
关键词
CD20; Skeletal muscle; Ca2+; SOCE
资金
- Associazione Amici del Centro Dino Ferrari
- Association Monegasque contre les Myopathies (AMM)
- Duchenne Parent Project de France (DPP France) [223098]
- Associazione La Nostra Famiglia Fondo DMD Gli Amici di Emanuele
Among the scarce available data about the biological role of the membrane protein CD20, there is some evidence that this protein functions as a store-operated Ca2+ channel and/or regulates transmembrane Ca2+ trafficking. Recent findings indicate that store-operated Ca2+ entry (SOCE) plays a central role in skeletal muscle function and development, but there remain a number of unresolved issues relating to SOCE modulation in this tissue. Here we describe CD20 expression in skeletal muscle, verifying its membrane localization in myoblasts and adult muscle fibers. Additionally, we show that inhibition of CD20 through antibody binding or gene silencing resulted in specific impairment of SOCE in C2C12 myoblasts. Our results provide novel insights into the CD20 expression pattern, and suggest that functional CD20 is required for SOCE to consistently occur in C2C12 myoblasts. These findings may contribute to future identification of mechanisms and molecules involved in the fine regulation of store-operated Ca2+ entry in skeletal muscle. (C) 2012 Elsevier Ltd. All rights reserved.
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