期刊
INTERNATIONAL JOURNAL OF ANTIMICROBIAL AGENTS
卷 36, 期 5, 页码 441-446出版社
ELSEVIER
DOI: 10.1016/j.ijantimicag.2010.06.034
关键词
Amphotericin; Biofilms; Candida albicans; Caspofungin; Tigecycline; Fluconazole
资金
- Department of Veterans' Affairs
- Biomedical Research Institute of New Mexico
Despite growing data on antimicrobial lock therapy (ALT) in treating bacterial catheter-related bloodstream infections (CR-BSIs), ALT has not been established as a treatment option for CR-BSI caused by Candida albicans. Based on our finding that high-dose doxycycline exhibited antifungal activity against mature C. albicans biofilms, we evaluated additional antibacterial agents with Gram-positive activity [azithromycin, tigecycline (TIG) and vancomycin]. After screening these antibiotics, it was found that TIG had substantial antifungal activity against mature C. albicans biofilms. Therefore, TIG was assayed alone and in combination with fluconazole (FLC), amphotericin B (AmB) or caspofungin (CAS). TIG at 2048 mu g/mL resulted in a >50% reduction in the growth of planktonic C. albicans cells. TIG inhibited the formation of biofilms from 128 mu g/mL. Against mature biofilms, 2048 mu g/mL TIG reduced metabolic activity by 84.2%. Furthermore, addition of 512 mu g/mL TIG to FLC at all concentrations tested provided additional reduction in the metabolic activity of mature biofilms. However, this was not superior to 512 mu g/mL TIG alone. TIG at 512 mu g/mL increased the antifungal effect of lower concentrations of AmB (0.03125-0.25 mu g/mL), but at 0.03125 mu g/mL and 0.0625 mu g/mL this effect was not superior to 512 mu g/mL TIG alone. TIG inhibited the antifungal effect of higher concentrations of AmB (>= 2 mu g/mL). TIG at 512 mu g/mL inhibited the antifungal activity of CAS at lower concentrations (0.25-8 mu g/mL). These data indicate that high-dose TIG is highly active in vitro against planktonic cells, forming biofilms and mature biofilms of C. albicans. Published by Elsevier B.V. on behalf of International Society of Chemotherapy.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据