期刊
INTERNATIONAL JOURNAL OF ANTIMICROBIAL AGENTS
卷 36, 期 3, 页码 234-238出版社
ELSEVIER
DOI: 10.1016/j.ijantimicag.2010.03.028
关键词
RND-type efflux pumps; Pseudomonas aeruginosa; Tetraphenylphosphonium; Outer membrane permeability
资金
- Finnish Center of Excellence, Academy of Finland [1129684]
- Lithuanian State Science and Studies Foundation [C-01/2009]
- COST action [BM0701]
Multidrug resistance (MDR) pumps are one of the major causes of antibiotic resistance in Pseudomonas aeruginosa. Thus, fast and reliable methods are needed to assay the efficiency of MDR pumps in these bacteria. In this study, it was demonstrated that a membrane voltage (Delta psi) indicator tetraphenylphosphonium (TPP+) in combination with the efflux pump inhibitor phenylalanyl-arginyl-beta-naphthylamide can be used to monitor the activity of resistance-nodulation-cell division (RND)-type efflux pumps in P. aeruginosa. By controlling the outer membrane permeability and Delta psi, electrochemical measurements of RND pump activity in real time were performed. It was demonstrated that the composition of the medium, the presence of nutrients and the level of aeration affect the efficiency of the TPP+-extruding activity of P. aeruginosa, urging the standardisation of experimental conditions to obtain quantitative and comparative results. (C) 2010 Elsevier B.V. and the International Society of Chemotherapy. All rights reserved.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据