期刊
INTERNATIONAL JOURNAL OF ANTIMICROBIAL AGENTS
卷 31, 期 5, 页码 458-462出版社
ELSEVIER
DOI: 10.1016/j.ijantimicag.2007.12.015
关键词
bacterial efflux pumps; efflux pump inhibitors; ethidium bromide; fluorometry; Escherichia coli; automated method
Demonstration of efflux of ethidium bromide (EtBr) has been made for over 30 bacterial species, usually by showing enhanced efflux in multidrug-resistant strains that was then abolished by inactivating efflux pumps. Here we present a relatively simple automated method that employs EtBr as an efflux pump substrate for the demonstration of intrinsic efflux activity in Escherichia coli K-12 AG100. The method uses the Rotor-Gene 3000 (TM) instrument for real-time fluorometric measurement of EtBr accumulation under conditions that limit energy (absence of glucose, low temperature) and of EtBr extrusion under optimum conditions. The method can be used for screening compound libraries for efflux inhibiting capacity. (C) 2008 Elsevier B.V. and the International Society of Chemotherapy. All rights reserved.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据