期刊
INTERNATIONAL JOURNAL FOR PARASITOLOGY
卷 44, 期 11, 页码 819-826出版社
ELSEVIER SCI LTD
DOI: 10.1016/j.ijpara.2014.06.007
关键词
Neuropeptide; G protein-coupled receptor; GPCR; Hindgut; Excretion
类别
资金
- National Institutes of Health, USA [R01AI090062]
The hindgut, as a part. of the tick excretory system, plays an important physiological role in maintaining homoeostases and waste elimination. Immunoreactive projections from the synganglion to the hindgut were found using antibodies against four different neuropeptides: FGLamide related allatostatin, myoinhibitory peptide, SIFamide, and orcokinin. The presence of FGLamide related allatostatin, myoinhibitory peptide and SIFamide in both synganglia (source) and hindgut (target organ) extracts was confirmed by MALDI-TOF. Tissue-specific PCR revealed the expression of four putative FGLamide related allatostatin receptors and an SIFamide receptor in the hindgut. An antibody against Ixodes scapularis SIFamide receptor detected immunoreactive spots in epithelial cells as well as the visceral muscles surrounding the rectal sac, while staining with the antibody against myoinhibitory peptide receptor I revealed that the immunoreactivity was only associated with the visceral muscles. In hindgut motility assays, SIFamide activated hindgut motility in a dose-dependent manner. None of other three neuropeptides (FGLamide related allatostatin, myoinhibitory peptide and orcokinin) activated hindgut motility when tested alone. Myoinhibitory peptide antagonised the SIFamide-stimulated hindgut mobility when it was tested in combination with SIFamide. Published by Elsevier Ltd. on behalf of Australian Society for Parasitology Inc.
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