期刊
INTERNATIONAL JOURNAL FOR PARASITOLOGY
卷 40, 期 1, 页码 45-54出版社
ELSEVIER SCI LTD
DOI: 10.1016/j.ijpara.2009.07.005
关键词
RNA editing; ATP synthase; Mitochondrion; Trypanosoma; Respiratory complex; Membrane potential
类别
资金
- Grant Agency of the Czech Republic [204/06/1558]
- Grant Agency of the Czech Academy of Sciences [A500960705]
- Czech Ministry of Education [LC07032, 2B06129, 6007665801]
- Slovak Ministry of Education
- Slovak Academy of Sciences [1/3241/06, 1/0393/09]
Throughout eukaryotes, the gene encoding subunit 6 (ATP6) of the F1FO-ATP synthase (complex V) is maintained in mitochondrial (mt) genomes, presumably because of its high hydrophobicity due to its incorporation into the membrane-bound F-O moiety. In Trypanosoma species, a mt transcript that undergoes extensive processing by RNA editing has a very low sequence similarity to ATP6 from other organisms. The notion that the putative ATP6 subunit is assembled into the F-O sub-complex is ostensibly challenged by the existence of naturally occurring dyskinetoplastic (Dk) and akinetoplastid (Ak) trypanosomes, which are viable despite lacking the mtDNA required for its expression. Taking advantage of the different phenotypes between RNA interference knock-down cell lines in which the expression of proteins involved in mtRNA metabolism and editing can be silenced, we provide support for the view that ATP6 is encoded in the mt genome of Trypanosoma species and that it is incorporated into complex V. The reduction of the F1FO oligomer of complex V coincides with the accumulation of the F, moiety in ATP6-lacking cells, which also appear to lack the F-O ATP9 multimeric ring. The oligomycin sensitivity of ATPase activity of complex V in ATP6-lacking cells is reduced, reflecting the insensitivity of the Dk and Ak cells to this drug. In addition, the F, moiety of complex V appears to exist as a dimer in steady state conditions and contains the ATP4 subunit traditionally assigned to the F-O sub-complex. (C) 2009 Australian Society for Parasitology Inc. Published by Elsevier Ltd. All rights reserved.
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