期刊
INTERNATIONAL JOURNAL FOR PARASITOLOGY
卷 38, 期 2, 页码 149-155出版社
PERGAMON-ELSEVIER SCIENCE LTD
DOI: 10.1016/j.ijpara.2007.10.007
关键词
Fasciola; newly excysted juvenile; cathepsin L; cathepsin B; RNA interference; tissue penetration
类别
资金
- NIAID NIH HHS [R01-AI49162] Funding Source: Medline
Probing protein function in parasitic flatworms is hampered by the difficulties associated with the development of transgenic approaches. Although RNA interference (RNAi) in schistosomes shows much promise, it has not been reported in other trematodes. Here, we show the successful silencing of the cysteine proteases cathepsin B and L in the infective stage of Fasciola hepatica newly excysted juveniles (NEJs). Silencing resulted in marked reductions in target transcript levels and significant diminution in the encoded proteins in the gut. RNAi of either enzyme in NEJs induced transient, abnormal locomotory phenotypes, and significantly reduced penetration of the rat intestinal wall. (c) 2007 Australian Society for Parasitology Inc. Published by Elsevier Ltd. All rights reserved.
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