Traditional medicine alpinetin inhibits the inflammatory response in Raw 264.7 cells and mouse models

Alpinetin, one of the main constituents of the seeds of Alpinia katsumadai Hayata, belonging to flavonoids, has been known to exhibit antibacterial, anti-inflammatory and other important therapeutic activities. The purpose of this study was to investigate the protection of alpinetin on inflammation in Lipopolysaccharide (LPS) stimulated Raw 264.7 cells and LPS induced vivo lung injury model. The effects of alpinetin on pro-inflammatory cytokines and signaling pathways were analyzed by enzyme-linked immunosorbent assay and Western blot The results showed that alpinetin markedly inhibited the LPS- induced TNF-alpha, IL-6 and IL-1 beta production both in vitro and vivo. Furthermore, alpinetin blocked the phosphorylation of I kappa B alpha protein, p65, p38 and extracellular signal-regulated kinase (ERK) in LPS stimulated RAW 264.7 cells. From in vivo study, it was also observed that alpinetin attenuated lung histopathologic changes in mouse models. These results suggest that alpinetin potentially decreases the inflammation in vitro and vivo, and might be a therapeutic agent against inflammatory diseases. (C) 2011 Elsevier B.V. All rights reserved.