期刊
INTERNATIONAL IMMUNOPHARMACOLOGY
卷 14, 期 4, 页码 471-480出版社
ELSEVIER SCIENCE BV
DOI: 10.1016/j.intimp.2012.08.021
关键词
Anti-inflammation; COX-2; Ear edema; iNOS; Myagropsis myagroides; NF-kappa B
资金
- National Fisheries Research and Development Institute [2012-12]
- Brain Busan [21]
Myagropsis myagroides has been used as a Chinese medicine and its extract has shown various biological activities, however, its anti-inflammatory mechanism remains unknown. In this study, we investigated the inhibitory effects of the ethyl acetate fraction of M. myagroides (EFM) on the production of inflammatory mediators and pro-inflammatory cytokines in lipopolysaccharides (LPS)-stimulated RAW 264.7 cells. EFM significantly inhibited LPS-induced production of nitric oxide (NO), prostaglandin E-2, and pro-inflammatory cytokines in a dose-dependent manner and suppressed the production of inducible nitric oxide synthase (iNOS) and cyclooxygenase-2 in RAW 264.7 cells. Inhibitory effect of EFM on iNOS expression and NO production was further confirmed using LPS-activated mouse peritoneal macrophages. EFM treatment strongly suppressed the activation of nuclear factor-kappa B (NF-kappa B) by suppressing phosphorylation of Akt and extracellular signal-regulated kinases (ERKs). EFM as well as phlorofucofuroeckol B (PFF-B), a major compound isolated from EFM, reduced ear edema induced by phorbol 12-myristate 13-acetate in mice. These results indicate that the anti-inflammatory effect of EFM, rich in PFF-B, on LPS-stimulated macrophages is regulated by the inhibition of NF-kappa B pathway through the inhibition of ERKs and Akt phosphorylation in LPS-stimulated macrophage cells. (C) 2012 Elsevier B.V. All rights reserved.
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