Tetrandrine suppresses amyloid-β-induced inflammatory cytokines by inhibiting NF-κB pathway in murine BV2 microglial cells

Microglial cells play an important role in mediating neuroinflammation in Alzheimer's disease (AD) by production of a series of proinflammatory mediators and clearance of A beta peptides and senile plaques. Tetrandrine, a bisbenzylisoquinoline alkaloid isolated from the Chinese herb Radix Stephania tetrandra, has been demonstrated to decrease the expression of proinflammatory mediators by inhibition of NF-kappa B activation. Here we investigated whether tetrandrine may affect the phagocytosis of microglia and the expression of cytokines and NF-kappa B in murine BV2 microglial cells. We found that fibrillar Amyloid-beta (fA beta) induced phagocytosis of microglia and dramatically increased the levels of interleukin 1 beta (IL-1 beta) and tumor necrosis factor alpha (TNF-alpha) as well as the expression of phospho NF-kappa B p65 in microglia cultures. The treatment with tetrandrine resulted in downregulation of phospho NF-kappa B p65 expression and strikingly reduced the production of IL-1 beta and TNF-alpha. However. tetrandrine did not affect fA beta induced phagocytosis of microglia. in conclusion, tetrandrine can decrease microglial detriment of neurotoxicity while maintaining microglial benefit of neuroprotection. Tetrandrine may be an efficacious and promising remedy in the treatment of AD. (C) 2011 Elsevier B.V. All rights reserved.