期刊
INTERNATIONAL IMMUNOPHARMACOLOGY
卷 11, 期 11, 页码 1954-1959出版社
ELSEVIER SCIENCE BV
DOI: 10.1016/j.intimp.2011.08.004
关键词
Thymic stromal lymphopoietin; Berberine; Nuclear factor-kappa B; Caspase-1
资金
- National Research Foundation of Korea (NRF)
- Ministry of Education, Science and Technology [2010-0005591]
- Kyung Hee University [KHU-20110092]
- National Research Foundation of Korea [2010-0005591] Funding Source: Korea Institute of Science & Technology Information (KISTI), National Science & Technology Information Service (NTIS)
Thymic stromal lymphopoietin (TSLP) plays a pivotal role in allergic diseases such as atopic dermatitis, asthma, and chronic obstructive pulmonary disease. However, it has not been clarified the effect of berberine (BER) on the production of TSLP yet. Thus, we investigated how BER inhibits the production of TSLP in the human mast cell line (HMC-1) cells. We used enzyme-linked immunosorbent assay, reverse transcription-polymerase chain reaction, luciferase assay, and Western blot analysis to investigate the effects of BER. BER inhibited the production and mRNA expression of TSLP in HMC-1 cells. BER also inhibited the nuclear factor-kappa B luciferase activity induced by phorbol myristate acetate plus A23187. BER inhibited the activation of caspase-1 in HMC-1 cells. Furthermore, BER inhibited the production of TSLP in primary mast cells. These results provide evidence that BER can help to treat inflammatory and atopic diseases through the inhibition of TSLP. (C) 2011 Elsevier B.V. All rights reserved.
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