期刊
INTERNATIONAL IMMUNOPHARMACOLOGY
卷 11, 期 10, 页码 1620-1627出版社
ELSEVIER
DOI: 10.1016/j.intimp.2011.05.024
关键词
Proanthocyanidins from grape seeds; Ulcerative colitis; Recurrent; Antiinflammatory; Tumor necrosis factor-alpha; Nuclear factor-kappa B
资金
- Natural Science Foundation of Gansu Province [3ZS061-A25-083]
- Natural Science Foundation of China [30672490]
The aim of this study was to elucidate the molecular mechanisms involved in the therapeutic effects of proanthocyanidins from grape seeds (GSPE) on recurrent ulcerative colitis (UC) in rats. GSPE in doses of 100, 200, and 400 mg/kg were intragastrically administered per day for 7 days after recurrent colitis was twice-induced by TNBS. The levels of GSH, as well as the activity of GSH-Px and SOD in colon tissues were measured by biochemical methods. The expression levels of tumor necrosis factor-alpha (TNF-alpha) and the nuclear translocation levels of nuclear factor-kappa B (NF-kappa B) in the colon tissues were measured by enzyme-linked immunosorbent assay methods. Western blotting analysis was used to determine the protein expression levels of inhibitory kappa B-alpha (tau), inhibitor kappa B kinase (IKK alpha/beta), phosphorylated I kappa B alpha and phosphorylated IKK alpha/beta. GSPE treatment was associated with a remarkable increased the activity of GSH-Px and SOD with GSH levels in TNBS-induced recurrent colitis rats as compared to the model group. GSPE also significantly reduced the expression levels of TNF-alpha, p-IKK alpha/beta, p-I kappa B alpha and the translocation of NF-kappa B in the colon mucosa. GSPE exerted a protective effect on recurrent colitis in rats by modifying the inflammatory response and promoting damaged tissue repair to improve colonic oxidative stress. Moreover, GSPE inhibited the TNBS-induced inflammatory of recurrent colitis though blocking NF-kappa B signaling pathways. (C) 2011 Elsevier B.V. All rights reserved.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据