期刊
INTERNATIONAL IMMUNOPHARMACOLOGY
卷 11, 期 8, 页码 1074-1078出版社
ELSEVIER
DOI: 10.1016/j.intimp.2011.03.001
关键词
Regulatory Tr1 cells; Immunotherapy; Rheumatoid arthritis
资金
- French ministry of Economy, Industry and Employment
- Conseil General des Alpes-Maritimes
- Provence-Alpes-Cote d'Azur (PACA) region
IL-10 producing regulatory type 1 (Tr1) cells represents a subpopulation of CD4+ regulatory cells able to prevent in vitro bystander T-cell proliferation and to inhibit a wide range of inflammatory diseases in mice. Our aim was to evaluate the frequency and function of joint specific Tr1 cells in the peripheral blood of severe Rheumatoid Arthritis (RA) patients. The collagen II protein was chosen to isolate Tr1 cells specific for a joint antigen. We successfully isolated In clones from 9 out of 11 RA patients. We showed that cells from patients display the same phenotype and surface marker regulation as previously shown for human Tr1 cells, characterized by expression of markers of regulation (FoxP3, CD25) at the activated but not at the resting state. Importantly, cells from patients showed Tr1 cytokine secretion (IL-10 and IFN-gamma) and immunosuppressive action on bystander T cell proliferation. Based on these results, we demonstrated that collagen II specific In cells can be isolated from the blood of severe refractory patients and that these cells are not altered in their phenotype and function. (C) 2011 Elsevier B.V. All rights reserved.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据