期刊
INTERNATIONAL IMMUNOPHARMACOLOGY
卷 9, 期 6, 页码 774-780出版社
ELSEVIER SCIENCE BV
DOI: 10.1016/j.intimp.2009.03.001
关键词
Osteoclastogenesis; Caffeic acid phenethyl ester; NF kappa B; NFATc1; c-Fos
资金
- Frontier Functional Proteomics Project [FPR08B1-170]
- New Drug Target Discovery [M10748000257-07N4800-25710]
- Science Research Center [R11-2008-023-01001-0]
Osteoclasts are multinuclear cells of myeloid lineage responsible for bone resorption. The anti-inflammatory property of caffeic acid phenethyl ester (CAPE), an active component of the propolis of honeybee hives, has been revealed. Since the regulatory mechanism of differentiation and activation of osteoclasts shares many well-known signaling pathways with that of inflammation, we investigated whether CAPE has any effect on osteoclastogenesis. CAPE potently suppressed osteoclastogenesis in cultures of bone marrow-derived precursor cells with the osteoclast differentiation factor, receptor activator of nuclear factor kappa B ligand (RANKL). While the RANKL-stimulated activation of the ERK JNK, and p38 MAPK signaling pathways was not affected, the DNA binding and transcription activity of NF kappa B were reduced by CAPE treatment. In addition, CAPE blocked the induction of NFATc1 and c-Fos following RANKL stimulation. Forced expression of c-Fos could reverse the inhibitory effect of CAPE on osteoclastogenesis. Finally, CAPE significantly inhibited the RANKL-induced osteoclast formation in mouse calvariae in vivo. We propose that CAPE might be useful as a therapeutic agent for treatment of bone destructive diseases. (C) 2009 Elsevier B.V. All rights reserved.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据