期刊
INTERNATIONAL DAIRY JOURNAL
卷 35, 期 1, 页码 32-37出版社
ELSEVIER SCI LTD
DOI: 10.1016/j.idairyj.2013.10.007
关键词
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资金
- National High Technology Research and Development Program of China [2011AA100901]
- National Key Technologies Program of China [2013BAD18B01]
The aim of this study was to investigate the diversity of phages from Chinese yoghurt plants. Restriction analysis of six isolated Streptococcus thermophilus (St) phages showed that Phi 101, Phi 102 and Phi 108 with different origins but with the same host strain St1 were different while phages Phi 901, Phi 903 and Phi 907 with host strain St9 from the same origin were homologous. DNA packaging mechanism analysis proved the isolated phages to be pac-type except Phi 108. The sensitive strain St1 could be infected by two different packaging mechanisms. Probing and sequencing of the variable region (VR2) fragment revealed that VR2 in aggressive phages (Phi 101, Phi 102 and Phi 108) infecting host strain St1 was deleted. Phage Phi 901 has an 845 bp amplicon and the similarity coefficient of the sequence was lower than 96% compared with other reported phages, which shows phage Phi 901 did not belong to any known grouping. (C) 2013 Elsevier Ltd. All rights reserved.
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