期刊
INTERNATIONAL DAIRY JOURNAL
卷 19, 期 9, 页码 531-536出版社
ELSEVIER SCI LTD
DOI: 10.1016/j.idairyj.2009.03.002
关键词
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资金
- Projects Consolider Ingenio 2010 FUNC-FOOD (Ministerio de Educacion y Ciencia) [CSD2007-00063]
- ANALISYC (Comunidad de Madrid) [S-505/AGR/0312]
- PIF-SIALOBIOTIC (CSIC) [200870F0101]
Four different fractionation techniques for obtaining galactooligosaccharides (GOS) free from digestible carbohydrates were compared. Diafiltration did not show any selectivity among mono-, di- and oligosaccharides, whereas yeast treatment to remove monosaccharides allowed a high recovery of GOS and disaccharides. Activated charcoal treatment showed a different selectivity in GOS recovery depending on the concentration of ethanolic water solutions used (1-15%). Ethanolic solutions of 8% led to a high recovery of GOS (similar to 90%), but 20% of disaccharides were also recovered, whilst solutions with 10% ethanol allowed almost complete removal of disaccharides, but only similar to 53% of GOS trisaccharides were recovered. Finally, the purest GOS fractions (degree of polymerization up to 8) were obtained using size exclusion chromatography. (C) 2009 Elsevier Ltd. All rights reserved.
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