期刊
INTERNATIONAL BIODETERIORATION & BIODEGRADATION
卷 82, 期 -, 页码 166-172出版社
ELSEVIER SCI LTD
DOI: 10.1016/j.ibiod.2013.03.008
关键词
Keratinolytic enzyme; Keratin waste; Purification; Biodegradation
资金
- National Natural Science Foundation of China [30900013, 20836003]
- National Key Technology R&D Program in the 12th Five year Plan of China [2011BAK10B03]
- National High Technology Research and Development Program of China (863 Program) [2011AA100905]
- Self-determined Research Program of Jiangnan University [JUSRP 21009]
Keratin-degrading strain Stenotrophomonas maltophilia BBE11-1 was isolated from a poultry farm. According to zymogram analysis, there were at least two keratinolytic enzymes produced. After three steps of purification by different chromatographies, three keratinolytic enzymes were effectively separated. Partial characterizations of three enzymes showed that K1, K2 and K3 were 48 kDa, 36 kDa and 17 kDa respectively. Alkaline pHs 7-11 and temperatures 40-50 degrees C were optimal for three enzymes. K3 protein cooperated with other two enzymes greatly enhanced keratinolytie activity and markedly degraded feather within 24 h. Cooperative action of K1 and K2 remarkably wiped off cuticle layers of wool without damaging internal fibers, and the addition of K3 with K1 and K2 also showed obvious structure change of wool. Cooperative mechanism of keratinous degradation and potential applications of keratin-waste hydrolysis or dehairing in leather industry by three enzymes were discussed. (C) 2013 Elsevier Ltd. All rights reserved.
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