期刊
INTERNATIONAL BIODETERIORATION & BIODEGRADATION
卷 65, 期 1, 页码 248-252出版社
ELSEVIER SCI LTD
DOI: 10.1016/j.ibiod.2010.11.008
关键词
Aspergillus; Co-cultivation; Polysaccharide degradation; Lignin degradation; Enzyme production
资金
- Utrecht University
- University of Hong Kong
- Dutch Foundation for Applied Science (STW) [07938, 07063]
Aspergillus niger and Aspergillus oryzae were co-cultivated with each other and with Magnaporthe grisea or Phanerochaete chrysosporium, respectively. Enzyme assays for plant polysaccharide and lignin-degrading enzymes showed that co-cultivation can improve extracellular enzyme production. Highest beta-glucosidase, alpha-cellobiohydrolase, beta-galactosidase, and laccase activities were found for A. oryzae in combination with other fungi, in particular with P. chrysosporium. Highest beta-xylosidase activity was obtained when A. niger was co-cultivated with P. chrysosporium. SDS-PAGE protein profiles demonstrated that A. niger and A. oryzae contributed most to the overall enzyme activities found in the culture medium of the mixed cultivations. These data demonstrate that co-cultivation of two major industrial fungi, A. niger and A. oryzae, results in improved production of biotechnologically relevant enzymes. (C) 2010 Elsevier Ltd. All rights reserved.
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