期刊
INTERNATIONAL BIODETERIORATION & BIODEGRADATION
卷 65, 期 1, 页码 85-91出版社
ELSEVIER SCI LTD
DOI: 10.1016/j.ibiod.2010.09.006
关键词
Hg-resistant bacteria; Pseudomonas; Psychrobacter; Hg2+ volatilization; Biofilm; Hg-bioremediation
资金
- Higher Institute for Environmental Protection and Research (ISPRA)
- Fondazione Monte dei Paschi di Siena
This study was aimed to isolate Hg-resistant bacteria from contaminated sediments of the Orbetello Lagoon in Italy and to assess their possible use as biofilms in bioremediation processes. Enrichment cultures prepared from contaminated sediments in the presence of 0.05 mM of mercury and under aerobic conditions allowed the isolation of five heterotrophic bacterial strains. 16S rDNA gene sequencing assigned the isolated strains to the genera Pseudomonas and Psychrobacter. For the first time mercury-resistant bacterial strains belonging to the genus Psychrobacter were evidenced. Minimum inhibitory concentrations in the presence of HgCl2 and of CH3HgCl showed high levels of resistance. EC50 values for the isolated bacterial strains in the presence of HgCl2 and of CH3HgCl confirmed the adaptation to the metal. Hg-resistant strains ORHg1, ORHg4 and ORHg5 showed the capacity to volatilize inorganic and organic mercury to elemental mercury, and formed biofilms on pumice particles, and were shown to play a role in the removal of mercury from sediment leachates. This study reports isolation and characterization of new Hg-resistant bacterial strains and provides novel insight into their possible use in bioremediation processes of mercury polluted sediments. (C) 2010 Elsevier Ltd. All rights reserved.
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