期刊
INTEGRATIVE CANCER THERAPIES
卷 10, 期 1, 页码 92-100出版社
SAGE PUBLICATIONS INC
DOI: 10.1177/1534735410378660
关键词
cell differentiation; cell migration; largehead atractylodes rhizome; PI3K/AKT pathway; Ras/ERK pathway; sesquiterpenes
资金
- Shanghai key disciplines [Y0301]
- Modernization of Traditional Chinese Medicine
- Shanghai Science and Technology Commission [05DZ19742]
- Hong Kong Baptist University [FRG/08-09/II-30]
The aims of this study were to isolate sesquiterpene compounds from the largehead atractylodes rhizome (LAR) and to investigate their effects on B16 cancer cells. A total of 8 sesquiterpenes from LAR were identified, of which eudesm-4 (15), 7-diene-9 alpha, 11-diol (7) was isolated for the first time. All 8 compounds inhibited growth of B16 cells, and atractylenolide I (AT-I), atractylenolide II (AT-II), and atractylenolactam (ATR) were the most potent, with IC50 values of 76.46, 84.02, and 54.88 mu m., respectively. Monomer lactone or lactam structures in the 8 compounds appeared to be critical for their antiproliferative activities. In addition, AT-I, AT-II, and ATR could induce cell differentiation and inhibit cell migration. Western blot analysis indicated that 2 of the compounds, AT-I and AT-II, could inactivate ERK, where all 3 inhibited AKT activation, suggesting that Ras/ERK and PI3K/AKT signaling pathways are involved in the action mechanisms of the LAR sesquiterpene compounds.
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