期刊
INTEGRATIVE BIOLOGY
卷 3, 期 5, 页码 560-567出版社
ROYAL SOC CHEMISTRY
DOI: 10.1039/c0ib00129e
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资金
- National Institutes of Health [EB00262, EB08396, HL73305, GM74048]
- Center for Engineering Cells and Regeneration of the University of Pennsylvania
- National Science Foundation
- Institute of Regenerative Medicine at the University of Pennsylvania
- NATIONAL HEART, LUNG, AND BLOOD INSTITUTE [R01HL073305] Funding Source: NIH RePORTER
- NATIONAL INSTITUTE OF BIOMEDICAL IMAGING AND BIOENGINEERING [R01EB000262, R01EB008396] Funding Source: NIH RePORTER
- NATIONAL INSTITUTE OF GENERAL MEDICAL SCIENCES [R01GM074048] Funding Source: NIH RePORTER
While it is well known that individual integrins are critical mediators of cell behavior, recent work has shown that when multiple types of integrins simultaneously engage the ECM, cell functions are enhanced. However, it is not known how integrins spatially coordinate to regulate cell adhesion because no reliable method exists to segregate integrins on the cell membrane. Here, we use a microcontact printing-based strategy to pattern multiple ECMs that bind distinct integrins in order to study how integrins might interact. In our technique, proteins are first adsorbed uniformly to a poly(dimethyl siloxane) stamp, and then selectively de-inked.'' Our strategy overcomes several inherent limitations of conventional microcontact printing, including stamp collapse and limited functionality of the surface patterns. We show that integrins spatially segregate on surfaces patterned with multiple ECMs, as expected. Interestingly, despite spatial segregation of distinct integrins, cells could form adhesions and migrate across multicomponent surfaces as well as they do on single component surfaces. Together, our data indicate that although cells can segregate individual integrins on the cell surface to mediate ECM-specific binding, integrins function cooperatively to guide cell adhesion and migration.
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