期刊
INTEGRATIVE AND COMPARATIVE BIOLOGY
卷 52, 期 6, 页码 781-791出版社
OXFORD UNIV PRESS INC
DOI: 10.1093/icb/ics052
关键词
-
类别
资金
- NICHD NIH HHS [R13HD070622, R15HD060017] Funding Source: Medline
Kryptolebias marmoratus is a synchronous hermaphroditic vertebrate that utilizes an ovotestis for reproduction. This fish develops externally, is easy to maintain, and has about a 100-day life cycle, making it a desirable developmental genetic model organism. Here, we present a pilot zygotic mutant screen utilizing the common chemical mutagen, N-ethyl-N-nitrosourea (ENU) to establish genetics in this model species. Selection of clonal stocks and optimal conditions for mutagenizing this fish are presented and the types and frequencies of zygotic mutants are documented in comparison to other fish models. Kryptolebias marmoratus is an exemplar model organism that will complement future developmental genetic screens in vertebrates.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据